Cancer detection Liquid biopsy Surface enhanced Raman spectroscopy (SERS) Transitional cell carcinoma (TCC) Urine
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摘要

Canine lower urinary tract neoplasia is a clinically important disease process that has high mortality due to late stage diagnosis and poorly durable response to treatment. Non-invasive diagnostic techniques (e.g. dipstick test, urine cytology) currently have poor diagnostic value, while more invasive tests (e.g. cystoscopy and biopsy) are costly and often require general anesthesia. We have developed and herein describe a quantitative cytological analysis method based on the use of surface-enhanced Raman spectroscopy (SERS), for identifying cancerous transitional cells in urine using SERS biotags (SBTs) carrying the peptide PLZ4 (amino acid sequence cQDGRMGFc) that targets malignant transitional cells. By analyzing the ratio of the PLZ4-SBTs to an on board control we were able to show that transitional cells had significantly higher ratios (P < 0.05) in patients diagnosed with transitional cell carcinoma (TCC) than in healthy samples.

译文

犬下泌尿道肿瘤是一种临床上重要的疾病过程,由于晚期诊断和对治疗的持久反应不佳,死亡率高。非侵入性诊断技术 (e. g. 试纸试验、尿细胞检查) 目前诊断价值较差,而侵入性检查较多 (e. g. 膀胱镜检查和活检) 费用昂贵,通常需要全身麻醉。我们已经开发并在此描述了一种基于表面增强拉曼光谱 (SERS) 的定量细胞分析方法,用于使用 SERS 生物标记 (SBTs) 识别尿液中的癌变移行细胞携带靶向恶性移行细胞的肽 PLZ4 (氨基酸序列 cQDGRMGFc)。通过分析 PLZ4-SBTs 与船上控制的比率,我们能够表明移行细胞在诊断为移行细胞癌 (TCC) 的患者中有明显更高的比率 (p <-0.05) 而不是健康样本。

Urine Cytology

肾内泌尿 主要用于发现泌尿系统的恶性肿瘤 诊断方式
概述  :  

尿细胞学检查是用显微镜观察尿沉渣各种细胞并评估其意义的方法,主要用于发现泌尿系统的恶性肿瘤。采集的细胞可反映较大范围的病变,如肾盂、输尿管和膀胱的癌细胞均能在尿液细胞学涂片中检出。尿细胞学检查方法简便、无创、特异性高,是泌尿系统肿瘤诊断和术后随访的主要检查方法,有助于肿瘤的早期诊断。 尿红细胞检查 1.相差显微镜检查:应用相差显微镜观察尿中红细胞形态可以区分肾小球及非肾小球性血尿。将血尿分为三类:(1)均一红细胞血尿;(2)变形红细胞血尿;

Urine   英 /ˈjʊərɪn; ˈjʊəraɪn/   美 /ˈjʊrɪn/

释    义   n. 尿

例    句   Later this year the urine and blood samples from the Danish puberty study will be tested for this chemical.今年底,丹麦青春期研究中的尿以及血液样本将会接受这种化学物质的测试。

 

Cytology   英 /saɪˈtɒlədʒi/   美 /saɪˈtɑːlədʒi/

释    义   n. 细胞学

例    句   Thus, the construction of the brain and the cytology, biophysics, and biochemistry of its component neurons reflect its fundamental function—to mediate behavior.神经元的在大脑中的构建,以及它的细胞学、生物物理学和生物化学都表现着它作为大脑基础构件的基本功能,也正是这些功能在调节着行为。

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